In the Bible, Lot (לוט "Hidden, covered", Standard Hebrew Lot, Tiberian Hebrew Lôṭ; Qur’anic Arabic لوط Lūṭ) was the nephew of the patriarch, Abraham or Abram. He was the son of Abraham's brother Haran. According to Jewish midrash, Abraham took care of Lot after Haran was burned in a gigantic fire in which Nimrod, King of Babylon, tried to kill Abraham.

Lot accompanied Abraham in all his journeys and went down to Egypt with Abraham and his family. The midrash gives Lot much credit because, despite his desire for wealth, he did not inform Pharaoh of the secret of Sarah, Abraham's wife. When Abraham journeyed to the Land of Canaan at the command of God, Lot accompanied him.

Lot and Abraham were both wealthy shepherds so that there came a time when the land they used could not contain the flocks of both. At this time Abraham and Lot split, Lot going southeast to plains near the cities of Sodom and Gomorrah.

When God decided to overturn and destroy the five cities of the plain, God sent angels to rescue Lot and his family in the merit of Abraham. However it was conditional that they did not look back. However Lot's wife turned back to see the destruction of the city and was hence turned into a pillar of salt. Lot and his two daughters escaped to the hills. Hence the phrase Remember Lot's wife.

Later, his daughters slept with their father on two subsequent nights and became impregnated. The first son born was later called 'Moav' -meaning in Hebrew -from the father [meh-Av]. He was the patriarch of the nation known as Moab. The second was called Amon, in Hebrew meaning: 'From our nation' and later became the patriarch of the nation of Amon.