Yakushi-ji (薬師寺) is an ancient Buddhist temple of the Hosso sect in the city of Nara, Nara Prefecture, Japan. The main image is of Yakushi Nyorai, the Healing Buddha.

Founded in 680 A.D. in Kashihara, Yakushiji was moved to its present location when the capital moved to Nara in 710.

Yakushiji is one of the places in Nara that UNESCO has designated as a World Heritage Site.