John Chambers could be any of the following people:

• John Chambers , the creator of the R programming language and S programming language
• John Graham Chambers, who codified the "Marquess of Queensberry rules," upon which modern day boxing is based.
• John Chambers , a politician in the 18th and 19th century
• John Chambers , make-up guru who won a special Oscar for his work on "Planet of the Apes"
• John Chambers , the Bishop of Peterborough
• John Chambers, President and CEO of Cisco Systems
• Jonmichael Chambers , Creator of the programming language for Robocode and Java